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XBTYF regulates Hoxb4-dependent activation of the Wnt/β-catenin pathway and attenuates apoptosis in OGD/R-injured H9C2 cells. (A) EdU assay for assessing H9C2 cell proliferation, (B) Calcein-AM/PI for evaluating cell apoptosis. XBTYF: 0.8 mg/mL, <t>IWR-1:</t> 10 μM. * p < 0.05, ** p < 0.01, n = 3. Scale bar: 100 μm.
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XBTYF regulates Hoxb4-dependent activation of the Wnt/β-catenin pathway and attenuates apoptosis in OGD/R-injured H9C2 cells. (A) EdU assay for assessing H9C2 cell proliferation, (B) Calcein-AM/PI for evaluating cell apoptosis. XBTYF: 0.8 mg/mL, <t>IWR-1:</t> 10 μM. * p < 0.05, ** p < 0.01, n = 3. Scale bar: 100 μm.
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XBTYF regulates Hoxb4-dependent activation of the Wnt/β-catenin pathway and attenuates apoptosis in OGD/R-injured H9C2 cells. (A) EdU assay for assessing H9C2 cell proliferation, (B) Calcein-AM/PI for evaluating cell apoptosis. XBTYF: 0.8 mg/mL, <t>IWR-1:</t> 10 μM. * p < 0.05, ** p < 0.01, n = 3. Scale bar: 100 μm.
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XBTYF regulates Hoxb4-dependent activation of the Wnt/β-catenin pathway and attenuates apoptosis in OGD/R-injured H9C2 cells. (A) EdU assay for assessing H9C2 cell proliferation, (B) Calcein-AM/PI for evaluating cell apoptosis. XBTYF: 0.8 mg/mL, <t>IWR-1:</t> 10 μM. * p < 0.05, ** p < 0.01, n = 3. Scale bar: 100 μm.
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XBTYF regulates Hoxb4-dependent activation of the Wnt/β-catenin pathway and attenuates apoptosis in OGD/R-injured H9C2 cells. (A) EdU assay for assessing H9C2 cell proliferation, (B) Calcein-AM/PI for evaluating cell apoptosis. XBTYF: 0.8 mg/mL, IWR-1: 10 μM. * p < 0.05, ** p < 0.01, n = 3. Scale bar: 100 μm.

Journal: Frontiers in Immunology

Article Title: Hoxb4 upregulation by Xuan Bi Tong Yu Fang confers cardioprotection via repression of the Wnt/β-catenin pathway in myocardial ischemia-reperfusion injury

doi: 10.3389/fimmu.2026.1767595

Figure Lengend Snippet: XBTYF regulates Hoxb4-dependent activation of the Wnt/β-catenin pathway and attenuates apoptosis in OGD/R-injured H9C2 cells. (A) EdU assay for assessing H9C2 cell proliferation, (B) Calcein-AM/PI for evaluating cell apoptosis. XBTYF: 0.8 mg/mL, IWR-1: 10 μM. * p < 0.05, ** p < 0.01, n = 3. Scale bar: 100 μm.

Article Snippet: IWR-1 (HY-12238) was purchased from MCE (New Jersey, USA).

Techniques: Activation Assay, EdU Assay

WB analysis of Wnt/β-catenin pathway in H9C2 cells after OGD/R injury, showing the effect of XBTYF via Hoxb4 on Wnt/β-catenin pathway activation. XBTYF: 0.8 mg/mL, IWR-1: 10 μM. * p < 0.05, ** p < 0.01, n = 3.

Journal: Frontiers in Immunology

Article Title: Hoxb4 upregulation by Xuan Bi Tong Yu Fang confers cardioprotection via repression of the Wnt/β-catenin pathway in myocardial ischemia-reperfusion injury

doi: 10.3389/fimmu.2026.1767595

Figure Lengend Snippet: WB analysis of Wnt/β-catenin pathway in H9C2 cells after OGD/R injury, showing the effect of XBTYF via Hoxb4 on Wnt/β-catenin pathway activation. XBTYF: 0.8 mg/mL, IWR-1: 10 μM. * p < 0.05, ** p < 0.01, n = 3.

Article Snippet: IWR-1 (HY-12238) was purchased from MCE (New Jersey, USA).

Techniques: Activation Assay